Dualsystems Biotech AG

Dualsystems Biotech AG

Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are a biotech company specialising in elucidating protein-protein interaction in the living cells focussing at the cell membrane. We identify the targets of your extracellular ligands using the ligand-receptor capture LRC-TriCEPS™ technology. During the interaction of your ligand of interest and its unknown targets and off-targets the cells are still alive and the unknown targets are in their natural microenvironment.

Moreover, with Flow-TriCEPS™ (Prestest TriCEPS™) we provide a tool to use in flow cytometry to identify the best binding conditions for your ligand of interest without the need of a detection antibody. Flow-TriCEPS can also be used in functional assays to test if a TriCEPS coupled ligand is functionally active. Flow TriCEPS couples the ligand by using primary amines (N-Term and Lysines) and since it contains a biotin, can be detected by a streptavidin fluorophore conjugate using flow cytometry.

Flow TriCEPS Services/Kit


Detection of the binding of your ligand to the cells using TriCEPS V2.0

Identification of the targets using TriCEPS V2.0 in or at the cell membrane of your ligand (peptide, protein, Antibody, ADC or other primary amine containing molecules) requires that the cells used are expressing the unknown targets. TriCEPS V2.0 is the ideal tool to screen cell lines or primary cells to identify the best cells to use later in the LRC-TriCEPS target identification experiments. Dualsystems offers these pretests for cell selection as both service and kit. Needed for the experiment are the ligand of interest and the cell lines/ primary cells that are expected to express the unknown target. There is no need for a ligand specific antibody.

Used for:

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyse protein/peptide binding to cells

A conceptual new approach for ligand-receptor capture on the living cell

Ligand receptor capture LRC-TriCEPS™ is a conceptually new technology to analyse the protein interaction of your biologics. We can identify the targets of your protein, antibody and peptide at the cell surface. Dualsystems offers the LRC-TriCEPS™ technology as a service for fee model to the industry and academia. The technology enables the unbiased identification of cell surface targets and off-targets on the living cells without genetic manipulation. Ligand-receptor capture technology can be used for many types of orphan ligands, such as peptides, proteins, antibodies, engineered affinity binders and also for viruses.

Orphan ligands:

  • Extracellular proteins
  • Peptide ligands
  • Antibodies
  • Engineered affinity binders
  • Viruses

Did you see our video interview about “LRC-TriCEPS™ Technology” that describes ligand-receptor capture (LRC) technology on the living cell?

Do you want to do functional pretests with your ligand coupled to LRC-TriCEPS™?