Posts

Target-identification

Target Identification publication at the EBR magazine April 2015

Target-identificationDo you need target identification of your ligand? Ligand-receptor capture (LRC-TriCEPSTM) a new approach to find the targets in the cell membrane on the living cells.

Read the article at the EBR magazine (site 25) for more details or the pdf.

EBR Magazin-April-15-Dualsystems-Ligand-receptor

LRC-blau-TriCEPS-02

Dualsystems Biotech AG

Dualsystems Biotech AG


Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are a biotech company specialising in elucidating protein-protein interaction in the living cells focussing at the cell membrane. We identify the targets of your extracellular ligands using the ligand-receptor capture LRC-TriCEPS™ technology. During the interaction of your ligand of interest and its unknown targets and off-targets the cells are still alive and the unknown targets are in their natural microenvironment.

Moreover, with Flow-TriCEPS™ (Prestest TriCEPS™) we provide a tool to use in flow cytometry to identify the best binding conditions for your ligand of interest without the need of a detection antibody. Flow-TriCEPS can also be used in functional assays to test if a TriCEPS coupled ligand is functionally active. Flow TriCEPS couples the ligand by using primary amines (N-Term and Lysines) and since it contains a biotin, can be detected by a streptavidin fluorophore conjugate using flow cytometry.

Flow TriCEPS Services/Kit



pretest-triceps-service-kit-yellow-flurescence1

Detection of the binding of your ligand to the cells using TriCEPS V2.0

Identification of the targets using TriCEPS V2.0 in or at the cell membrane of your ligand (peptide, protein, Antibody, ADC or other primary amine containing molecules) requires that the cells used are expressing the unknown targets. TriCEPS V2.0 is the ideal tool to screen cell lines or primary cells to identify the best cells to use later in the LRC-TriCEPS target identification experiments. Dualsystems offers these pretests for cell selection as both service and kit. Needed for the experiment are the ligand of interest and the cell lines/ primary cells that are expected to express the unknown target. There is no need for a ligand specific antibody.

Used for:

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyse protein/peptide binding to cells

A conceptual new approach for ligand-receptor capture on the living cell

Ligand receptor capture LRC-TriCEPS™ is a conceptually new technology to analyse the protein interaction of your biologics. We can identify the targets of your protein, antibody and peptide at the cell surface. Dualsystems offers the LRC-TriCEPS™ technology as a service for fee model to the industry and academia. The technology enables the unbiased identification of cell surface targets and off-targets on the living cells without genetic manipulation. Ligand-receptor capture technology can be used for many types of orphan ligands, such as peptides, proteins, antibodies, engineered affinity binders and also for viruses.

Orphan ligands:

  • Extracellular proteins
  • Peptide ligands
  • Antibodies
  • Engineered affinity binders
  • Viruses

Did you see our video interview about “LRC-TriCEPS™ Technology” that describes ligand-receptor capture (LRC) technology on the living cell?

Do you want to do functional pretests with your ligand coupled to LRC-TriCEPS™?

Swiss Biotech Day 2014, 8. April

The Swiss Biotech Day on 8 April 2014 is the leading biotechnology conference in Switzerland. Every year, this event brings together more than 200 senior executives from the life science industry across Europe.

For a face to face meeting to learn more about Dualsystems and its new technology to identify receptors of your ligand on living cells contact Dr. Paul Helbling via email paul.helbling@dualsystems.com or mobile +41 76 422 55 05

 

 

Sitemap

Sitemap

  • Home | Dualsystems Biotech is a provider of custom proteomics services for industry and academia, specialising in ligand-receptor capture, affinity purification/mass spectrometry analysis of protein complexes and stable cell line generation.In addition, Dualsystems provides kits and reagents for a range of research applications, including protein expression, protein-protein interaction and protein activity measurement.
  • LRC-TriCEPS Services | LRC-TriCEPS™ ligand-receptor capture Services
    • Overview | TriCEPS™-based identification of ligand-receptor interactions
    • Principle | Dualsystems identify binding partners for Ligand-receptor interaction
    • Literature | TriCEPS-based ligand-receptor capture
  • Affinity and receptor expression | Assessment of the limits of the LRC-TriCEPS™ technology
  • Cell Types | Living cell types used with LRC-TriCEPS
  • Example of LRC-TriCEPS applied to human primary T-cells
  • Example of a LRC-TriCEPS experiment
  • Flow (Pretest) TriCEPS™ Kit |
  • News | Get here the latest news about Dualsystems Biotech custom screening services and products.
  • Blog | Dualsystems blog
  • Events | Events, trade fairs, partnering events Dualsytems plans to attend.
  • Company | Dualsystems Biotech is a provider of custom services to identify protein-protein interactions, including yeast two-hybrid services and our proprietary DUALmembrane and DUALhunter technologies for the identification of interactions involving membrane proteins and transcription factors. In addition, we provide kits and reagents for a range of research applications, including protein expression, protein purification and protein activity measurement.
  • Management of Dualsystems | Find more information about the Dualsystems Biotech management team.
  • Scientific Board | Find here more information about the Dualsystems Biotech scientific advisory board.
  • Investors | Are you interested in investment opportunities with Dualsystems Biotech?
  • Career | Are you interested in working for Dualsystems Biotech?
  • Contact | Contact Dualsystems Biotech by email, telephone or our online contact form and we will get back to you within 24 hours!
  • Bioinformatics tools | Bioinformatics tools

Pages

LRC-blau-TriCEPS-02

Dualsystems Biotech AG

Dualsystems Biotech AG


Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are a biotech company specialising in elucidating protein-protein interaction in the living cells focussing at the cell membrane. We identify the targets of your extracellular ligands using the ligand-receptor capture LRC-TriCEPS™ technology. During the interaction of your ligand of interest and its unknown targets and off-targets the cells are still alive and the unknown targets are in their natural microenvironment.

Moreover, with Flow-TriCEPS™ (Prestest TriCEPS™) we provide a tool to use in flow cytometry to identify the best binding conditions for your ligand of interest without the need of a detection antibody. Flow-TriCEPS can also be used in functional assays to test if a TriCEPS coupled ligand is functionally active. Flow TriCEPS couples the ligand by using primary amines (N-Term and Lysines) and since it contains a biotin, can be detected by a streptavidin fluorophore conjugate using flow cytometry.

Flow TriCEPS Services/Kit



pretest-triceps-service-kit-yellow-flurescence1

Detection of the binding of your ligand to the cells using TriCEPS V2.0

Identification of the targets using TriCEPS V2.0 in or at the cell membrane of your ligand (peptide, protein, Antibody, ADC or other primary amine containing molecules) requires that the cells used are expressing the unknown targets. TriCEPS V2.0 is the ideal tool to screen cell lines or primary cells to identify the best cells to use later in the LRC-TriCEPS target identification experiments. Dualsystems offers these pretests for cell selection as both service and kit. Needed for the experiment are the ligand of interest and the cell lines/ primary cells that are expected to express the unknown target. There is no need for a ligand specific antibody.

Used for:

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyse protein/peptide binding to cells

A conceptual new approach for ligand-receptor capture on the living cell

Ligand receptor capture LRC-TriCEPS™ is a conceptually new technology to analyse the protein interaction of your biologics. We can identify the targets of your protein, antibody and peptide at the cell surface. Dualsystems offers the LRC-TriCEPS™ technology as a service for fee model to the industry and academia. The technology enables the unbiased identification of cell surface targets and off-targets on the living cells without genetic manipulation. Ligand-receptor capture technology can be used for many types of orphan ligands, such as peptides, proteins, antibodies, engineered affinity binders and also for viruses.

Orphan ligands:

  • Extracellular proteins
  • Peptide ligands
  • Antibodies
  • Engineered affinity binders
  • Viruses

Did you see our video interview about “LRC-TriCEPS™ Technology” that describes ligand-receptor capture (LRC) technology on the living cell?

Do you want to do functional pretests with your ligand coupled to LRC-TriCEPS™?

Sitemap

Sitemap

  • Home | Dualsystems Biotech is a provider of custom proteomics services for industry and academia, specialising in ligand-receptor capture, affinity purification/mass spectrometry analysis of protein complexes and stable cell line generation.In addition, Dualsystems provides kits and reagents for a range of research applications, including protein expression, protein-protein interaction and protein activity measurement.
  • LRC-TriCEPS Services | LRC-TriCEPS™ ligand-receptor capture Services
    • Overview | TriCEPS™-based identification of ligand-receptor interactions
    • Principle | Dualsystems identify binding partners for Ligand-receptor interaction
    • Literature | TriCEPS-based ligand-receptor capture
  • Affinity and receptor expression | Assessment of the limits of the LRC-TriCEPS™ technology
  • Cell Types | Living cell types used with LRC-TriCEPS
  • Example of LRC-TriCEPS applied to human primary T-cells
  • Example of a LRC-TriCEPS experiment
  • Flow (Pretest) TriCEPS™ Kit |
  • News | Get here the latest news about Dualsystems Biotech custom screening services and products.
  • Blog | Dualsystems blog
  • Events | Events, trade fairs, partnering events Dualsytems plans to attend.
  • Company | Dualsystems Biotech is a provider of custom services to identify protein-protein interactions, including yeast two-hybrid services and our proprietary DUALmembrane and DUALhunter technologies for the identification of interactions involving membrane proteins and transcription factors. In addition, we provide kits and reagents for a range of research applications, including protein expression, protein purification and protein activity measurement.
  • Management of Dualsystems | Find more information about the Dualsystems Biotech management team.
  • Scientific Board | Find here more information about the Dualsystems Biotech scientific advisory board.
  • Investors | Are you interested in investment opportunities with Dualsystems Biotech?
  • Career | Are you interested in working for Dualsystems Biotech?
  • Contact | Contact Dualsystems Biotech by email, telephone or our online contact form and we will get back to you within 24 hours!
  • Bioinformatics tools | Bioinformatics tools