Posts

LRC-blau-TriCEPS-02

Dualsystems Biotech AG

Dualsystems Biotech AG


Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are a biotech company specialized in elucidating protein-protein interaction in the living cells especially at the cell membrane. We identify the targets of your extracellular ligands using the ligand-receptor capture LRC-TriCEPS™ technology. During the interaction of your ligand of interest and its unknown targets and off-targets the cells are still alive and the unknown targets are in their natural microenvironment.

Further, with Flow-TriCEPS™ (Prestest TriCEPS™) we provide a tool to use in flow cytometry to identify the best binding conditions for your ligand of interest without the need of a detection antibody. Flow-TriCEPS can also be used in functional assays to test if TriCEPS coupled ligand is functionally active. Flow TriCEPS couples the ligand by using primary amines (N-Term and Lysines) and since it contains a biotin, can be detected by a streptavidin fluorophore conjugate using flow cytometry.

Flow TriCEPS Services/Kit



pretest-triceps-service-kit-yellow-flurescence1

Detection of the binding of your ligand to the cells using TriCEPS V2.0

Identification of the targets using TriCEPS V2.0 in or at the cell membrane of your ligand (peptide, protein, Antibody, ADC or other primary amine containing molecules) requires that the cells used are expressing the unknown targets. TriCEPS V2.0 is the ideal tool to screen cell lines or primary cells to identify the best cells to use later in the LRC-TriCEPS target identification experiments. Dualsystems offers these pretests for cell selection as service and kit. Needed for the experiment is the ligand of interest and the cell lines/ primary cells that are expected to express the unknown target. There is no need for a ligand specific antibody.

Used for:

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyze protein/peptide binding to cells

A conceptual new approach for ligand-receptor capture on the living cell

Ligand receptor capture LRC-TriCEPS™ is a conceptually new technology to analyse the protein interaction of your biologics. We can identify the targets of your protein, antibody and peptide at the cell surface. Dualsystems offers the LRC-TriCEPS™ technology as a service for fee model to the industry and academia. The technology enables the unbiased identification of cell surface targets and off-targets on the living cells without genetic manipulation. Ligand-receptor capture technology can be used for many types of orphan ligands, such as peptides, proteins, antibodies, engineered affinity binders but also for viruses.

Orphan ligands:

  • Extracellular proteins
  • Peptide ligands
  • Antibodies
  • Engineered affinity binders
  • Viruses

Did you see our Video Interview about LRC-TriCEPS™ Services, ligand-receptor capture technology on the living cell?

LRC-TriCEPS and Flow-TriCEPS Flyer

Do you want to do functional pretests with your ligand coupled to LRC-TriCEPS™?

Affinity and receptor expression limits tested for LRC-TriCEPS

Dualsystems Biotech AG is testing the limits of the LRC-TriCEPSTM (CaptiRec) technology using antibodies with different affinities against the same target and cells expressing the target receptor with different expression levels.

Limits-of-LRC-TriCEPS

Limits-of-LRC-TriCEPS

Details of the assesment see here

Dualsystems Biotech AG and Biaffin GmbH & Co KG perform together an example of an experiment

Dualsystems Biotech AG and Biaffin GmbH & Co KG  perform together an example of an experiment.

In the case study Dualsystems Biotech AG determined the targets of the Anti EGFR antibodies and Biaffin GmbH& Co KG  measured the binding characteristics of the ligand receptor interaction using Surface Plasmon Resonance (SPR).

 

Video how the LRC-TriCEPS™ technology works

Video – how the LRC-TriCEPS™ technology works

Dualsystems Biotech AG Press Release

How the LRC-TriCEPS™ technology works (Video)

Zürich Switzerland – June 23, 2014 – Dualsystems Biotech AG offers the most advanced technology for identifying receptor(s) for orphan ligands. The ligand-receptor capture technology (LRC-TriCEPS™) is an innovative and conceptually new approach for the unbiased discovery of cell surface interactions of ligands ranging from peptides, proteins, antibodies to viruses. Dualsystems offers the LRC-TriCEPS™ technology in our service line CaptiRec. We offer a variety of formats which will fit your needs: From CaptiRec Kits for your own in-house experiments to an all-inclusive CaptiRec Service.

Dualsystems Biotech AG is looking forward to support your project with the newest invention in receptor/target discovery.

Please watch the video where we explain how the LRC-TriCEPS™ technology works: LRC-TriCEPS™-Video

Video-Dualsystems-LRC-Triceps

How the LRC-TriCEPS™ technology works

Video how the LRC-TriCEPS™ technology works


LRC-TriCEPS™ (CaptiRec) is a novel approach which enables the identification of cell surface receptors and off-targets on the living cells for a wide range of orphan ligands, such as:

  • Peptides
  • Protein
  • Antibodies
  • Engineered affinity binders
  • Viruses

Watch the video to see how the LRC-TriCEPS™ technology works


Testimonials:

  • IGENICA BIOTHERAPEUTICS (USA)
  • University of California San Francisco (UCSF)
  • CEDOC – Centro de Estudos de Doenças Crónicas
  • Washington University School of Medicine

Dualsystems Biotech partners with IBR Inc. to validate the workflow of the CaptiRec Technology

Dualsystems Biotech AG Press Release

Dualsystems Biotech partners with IBR Inc. to validate the workflow of the CaptiRec Technology

Schlieren, Switzerland, January 2014: – Dualsystems Biotech AG has announced it will collaborate with another Swiss laboratory, Institute for Biopharmaceutical Research (IBR Inc.) to validate the CaptiRec Triceps/Ligand Receptor Capture Technology. This technology is used to identify the Cell Membrane Receptors on Living Cells.
The partnership will combine the respective strengths of Dualsystems, a leading service provider for protein interaction at the cell membrane and IBR Inc., a GLP-compliant contract research organization specializing in cell-based assays

Read more

Terms of use agreement

Terms of use agreement

Dualsystems Biotech AG
Grabenstrasse 11a
8952 Schlieren, Switzerland

Tel. +41 44 738 50 00
Fax +41 44 738 50 05

1. Third-Party Sites
This site may include links to other sites on the Internet that are owned and operated by online merchants and other third parties. Member acknowledges that Dualsystems is not responsible for the availability of, or the content located on or through, any third-party site. Member should contact the site administrator or webmaster for those third-party sites if Member has any concerns regarding such links or the content located on such sites. Members use of those third-party sites is subject to the terms of use and privacy policies of each site, and Dualsystems is not responsible for this content. We encourage all Members to review said privacy policies of third-parties’ sites.

2. Disclaimer Regarding Accuracy of Web Site Information
While Dualsystems Biotech AG makes every effort to ensure that the information on this website is accurate, we can make no representations or warranties as to the accuracy or reliability of any information provided on this website.

3. Copyright and Trademark Information
All content included or available on this site, including site design, text, graphics, interfaces, and the selection and arrangements thereof is the property of is the property of Dualsystems and/or third parties protected by intellectual property rights. Any use of materials on the website, including reproduction for purposes other than those noted above, modification, distribution, or replication, any form of data extraction or data mining, or other commercial exploitation of any kind, without prior written permission of an authorized officer of Dualsystems is strictly prohibited. Members agree that they will not use any robot, spider, or other automatic device, or manual process to monitor or copy our web pages or the content contained therein without prior written permission of an authorized officer of Dualsystems.

Dualsystems’s trademarks may not be used in connection with any product or service that is not provided by Dualsystems, in any manner that is likely to cause confusion among customers, or in any manner that disparages or discredits Dualsystems or it parent company.

All other trademarks displayed on Dualsystems’s website are the trademarks of their respective owners, and constitute neither an endorsement nor a recommendation of those Vendors. In addition, such use of trademarks or links to the web sites of Vendors is not intended to imply, directly or indirectly, that those Vendors endorse or have any affiliation with Dualsystems Biotech AG.

4. Botnets
Dualsystems Biotech AG retains the right, at our sole discretion, to terminate any accounts involved with botnets and related activities. If any hostnames are used as command and control points for botnets, Dualsystems reserves the right to direct the involved hostnames to a honeypot, loopback address, logging facility, or any other destination at our discretion.

5. Privacy
This website uses Google Analytics, a web analytics service provided by Google, Inc. (“Google”).  Google Analytics uses “cookies”, which are text files placed on your computer, to help the website analyze how users use the site. The information generated by the cookie about your use of the website (including your IP address) will be transmitted to and stored by Google on servers in the United States . Google will use this information for the purpose of evaluating your use of the website, compiling reports on website activity for website operators and providing other services relating to website activity and internet usage.  Google may also transfer this information to third parties where required to do so by law, or where such third parties process the information on Google’s behalf. Google will not associate your IP address with any other data held by Google.  You may refuse the use of cookies by selecting the appropriate settings on your browser, however please note that if you do this you may not be able to use the full functionality of this website.  By using this website, you consent to the processing of data about you by Google in the manner and for the purposes set out above.

6. Other Terms
If any provision of this Terms of Use Agreement shall be unlawful, void or unenforceable for any reason, the other provisions (and any partially-enforceable provision) shall not be affected thereby and shall remain valid and enforceable to the maximum possible extent. Member agrees that this Terms of Use Agreement and any other agreements referenced herein may be assigned by Dualsystems, in our sole discretion, to a third party in the event of a merger or acquisition. This Terms of Use Agreement shall apply in addition to, and shall not be superseded by, any other written agreement between Member and Dualsystems Biotech AG in relation to your participation as a Member. Member agrees that by accepting this Terms of Use Agreement, Member is consenting to the use and disclosure of their personally identifiable information and other practices described in our Privacy Policy Statement.

Sitemap

Sitemap

  • Home | Dualsystems Biotech is a provider of custom proteomics services for industry and academia, specializing in ligand-receptor capture, affinity purification/mass spectrometry analysis of protein complexes and stable cell line generation.In addition, Dualsystems provides kits and reagents for a range of research applications, including protein expression, protein-protein interaction and protein activity measurement.
  • LRC-TriCEPS Services | LRC-TriCEPS™ ligand-receptor capture Services
    • Overview | TriCEPS™-based identification of ligand-receptor interactions
    • Principle | Dualsystems identify binding partners for Ligand-receptor interaction
    • Literature | TriCEPS-based ligand-receptor capture
  • Affinity and receptor expression | Assessment of the limits of the LRC-TriCEPS™ technology
  • Cell Types | Living cell types used with LRC-TriCEPS
  • Example of LRC-TriCEPS applied to human primary T-cells
  • Example of a LRC-TriCEPS experiment
  • Flow (Pretest) TriCEPS™ Kit |
  • News | Get here the latest news about Dualsystems Biotech custom screening services and products.
  • Blog | Blog article from Dualsystems.
  • Events | Events, trade fairs, partnering events Dualsytems plans to attend.
  • Company | Dualsystems Biotech is a provider of custom services to identify protein-protein interactions, including yeast two-hybrid services and our proprietary DUALmembrane and DUALhunter technologies for the identification of interactions involving membrane proteins and transcription factors. In addition, we provide kits and reagents for a range of research applications, including protein expression, protein purification and protein activity measurement.
  • Management of Dualsystems | Find more information about the Dualsystems Biotech management team.
  • Scientific Board | Find here more information about the Dualsystems Biotech scientific advisory board.
  • Investors | Are you interested in investment opportunities with Dualsystems Biotech?
  • Career | Are you interested in working for Dualsystems Biotech?
  • Contact | Get in contact with Dualsystems Biotech. Contact us by email, telephone or our online contact form and we will come back to you within 24 hours!
  • Bioinformatics tools | Bioinformatics tools

Investors

Investors


During two financing rounds in 2000 and in 2007 Dualsystems Biotech has built a solid investor base including private individuals as well as institutional investors to support our technology driven growth strategy.

Should you be interested in supporting us and become an investor in Dualsystems Biotech, please contact Dr. Paul Helbling, at subject line Investment.

Company

About Dualsystems Biotech


Dualsystems Biotech Schlieren Zurich Switzerland

Dualsystems Biotech is a provider of proteomics services for industry and academia. The LRC TriCEPS technology platform is designed to identify targets and off-targets irected to identify intracellular as well as extracellular drug targets. Our specialities LRC-TriCEPS™, ligand-receptor capture and FLOW-TriCEPS™ make use of mass spectrometry analysis of protein complexes.

  • Established in 2000
  • Facilities in Zurich, Switzerland
  • Provider of target screening technologies
  • Swiss Quality

Merken

Merken

Merken

Pages

LRC-blau-TriCEPS-02

Dualsystems Biotech AG

Dualsystems Biotech AG


Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are a biotech company specialized in elucidating protein-protein interaction in the living cells especially at the cell membrane. We identify the targets of your extracellular ligands using the ligand-receptor capture LRC-TriCEPS™ technology. During the interaction of your ligand of interest and its unknown targets and off-targets the cells are still alive and the unknown targets are in their natural microenvironment.

Further, with Flow-TriCEPS™ (Prestest TriCEPS™) we provide a tool to use in flow cytometry to identify the best binding conditions for your ligand of interest without the need of a detection antibody. Flow-TriCEPS can also be used in functional assays to test if TriCEPS coupled ligand is functionally active. Flow TriCEPS couples the ligand by using primary amines (N-Term and Lysines) and since it contains a biotin, can be detected by a streptavidin fluorophore conjugate using flow cytometry.

Flow TriCEPS Services/Kit



pretest-triceps-service-kit-yellow-flurescence1

Detection of the binding of your ligand to the cells using TriCEPS V2.0

Identification of the targets using TriCEPS V2.0 in or at the cell membrane of your ligand (peptide, protein, Antibody, ADC or other primary amine containing molecules) requires that the cells used are expressing the unknown targets. TriCEPS V2.0 is the ideal tool to screen cell lines or primary cells to identify the best cells to use later in the LRC-TriCEPS target identification experiments. Dualsystems offers these pretests for cell selection as service and kit. Needed for the experiment is the ligand of interest and the cell lines/ primary cells that are expected to express the unknown target. There is no need for a ligand specific antibody.

Used for:

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyze protein/peptide binding to cells

A conceptual new approach for ligand-receptor capture on the living cell

Ligand receptor capture LRC-TriCEPS™ is a conceptually new technology to analyse the protein interaction of your biologics. We can identify the targets of your protein, antibody and peptide at the cell surface. Dualsystems offers the LRC-TriCEPS™ technology as a service for fee model to the industry and academia. The technology enables the unbiased identification of cell surface targets and off-targets on the living cells without genetic manipulation. Ligand-receptor capture technology can be used for many types of orphan ligands, such as peptides, proteins, antibodies, engineered affinity binders but also for viruses.

Orphan ligands:

  • Extracellular proteins
  • Peptide ligands
  • Antibodies
  • Engineered affinity binders
  • Viruses

Did you see our Video Interview about LRC-TriCEPS™ Services, ligand-receptor capture technology on the living cell?

LRC-TriCEPS and Flow-TriCEPS Flyer

Do you want to do functional pretests with your ligand coupled to LRC-TriCEPS™?

Video-Dualsystems-LRC-Triceps

How the LRC-TriCEPS™ technology works

Video how the LRC-TriCEPS™ technology works


LRC-TriCEPS™ (CaptiRec) is a novel approach which enables the identification of cell surface receptors and off-targets on the living cells for a wide range of orphan ligands, such as:

  • Peptides
  • Protein
  • Antibodies
  • Engineered affinity binders
  • Viruses

Watch the video to see how the LRC-TriCEPS™ technology works


Testimonials:

  • IGENICA BIOTHERAPEUTICS (USA)
  • University of California San Francisco (UCSF)
  • CEDOC – Centro de Estudos de Doenças Crónicas
  • Washington University School of Medicine

Terms of use agreement

Terms of use agreement

Dualsystems Biotech AG
Grabenstrasse 11a
8952 Schlieren, Switzerland

Tel. +41 44 738 50 00
Fax +41 44 738 50 05

1. Third-Party Sites
This site may include links to other sites on the Internet that are owned and operated by online merchants and other third parties. Member acknowledges that Dualsystems is not responsible for the availability of, or the content located on or through, any third-party site. Member should contact the site administrator or webmaster for those third-party sites if Member has any concerns regarding such links or the content located on such sites. Members use of those third-party sites is subject to the terms of use and privacy policies of each site, and Dualsystems is not responsible for this content. We encourage all Members to review said privacy policies of third-parties’ sites.

2. Disclaimer Regarding Accuracy of Web Site Information
While Dualsystems Biotech AG makes every effort to ensure that the information on this website is accurate, we can make no representations or warranties as to the accuracy or reliability of any information provided on this website.

3. Copyright and Trademark Information
All content included or available on this site, including site design, text, graphics, interfaces, and the selection and arrangements thereof is the property of is the property of Dualsystems and/or third parties protected by intellectual property rights. Any use of materials on the website, including reproduction for purposes other than those noted above, modification, distribution, or replication, any form of data extraction or data mining, or other commercial exploitation of any kind, without prior written permission of an authorized officer of Dualsystems is strictly prohibited. Members agree that they will not use any robot, spider, or other automatic device, or manual process to monitor or copy our web pages or the content contained therein without prior written permission of an authorized officer of Dualsystems.

Dualsystems’s trademarks may not be used in connection with any product or service that is not provided by Dualsystems, in any manner that is likely to cause confusion among customers, or in any manner that disparages or discredits Dualsystems or it parent company.

All other trademarks displayed on Dualsystems’s website are the trademarks of their respective owners, and constitute neither an endorsement nor a recommendation of those Vendors. In addition, such use of trademarks or links to the web sites of Vendors is not intended to imply, directly or indirectly, that those Vendors endorse or have any affiliation with Dualsystems Biotech AG.

4. Botnets
Dualsystems Biotech AG retains the right, at our sole discretion, to terminate any accounts involved with botnets and related activities. If any hostnames are used as command and control points for botnets, Dualsystems reserves the right to direct the involved hostnames to a honeypot, loopback address, logging facility, or any other destination at our discretion.

5. Privacy
This website uses Google Analytics, a web analytics service provided by Google, Inc. (“Google”).  Google Analytics uses “cookies”, which are text files placed on your computer, to help the website analyze how users use the site. The information generated by the cookie about your use of the website (including your IP address) will be transmitted to and stored by Google on servers in the United States . Google will use this information for the purpose of evaluating your use of the website, compiling reports on website activity for website operators and providing other services relating to website activity and internet usage.  Google may also transfer this information to third parties where required to do so by law, or where such third parties process the information on Google’s behalf. Google will not associate your IP address with any other data held by Google.  You may refuse the use of cookies by selecting the appropriate settings on your browser, however please note that if you do this you may not be able to use the full functionality of this website.  By using this website, you consent to the processing of data about you by Google in the manner and for the purposes set out above.

6. Other Terms
If any provision of this Terms of Use Agreement shall be unlawful, void or unenforceable for any reason, the other provisions (and any partially-enforceable provision) shall not be affected thereby and shall remain valid and enforceable to the maximum possible extent. Member agrees that this Terms of Use Agreement and any other agreements referenced herein may be assigned by Dualsystems, in our sole discretion, to a third party in the event of a merger or acquisition. This Terms of Use Agreement shall apply in addition to, and shall not be superseded by, any other written agreement between Member and Dualsystems Biotech AG in relation to your participation as a Member. Member agrees that by accepting this Terms of Use Agreement, Member is consenting to the use and disclosure of their personally identifiable information and other practices described in our Privacy Policy Statement.

Sitemap

Sitemap

  • Home | Dualsystems Biotech is a provider of custom proteomics services for industry and academia, specializing in ligand-receptor capture, affinity purification/mass spectrometry analysis of protein complexes and stable cell line generation.In addition, Dualsystems provides kits and reagents for a range of research applications, including protein expression, protein-protein interaction and protein activity measurement.
  • LRC-TriCEPS Services | LRC-TriCEPS™ ligand-receptor capture Services
    • Overview | TriCEPS™-based identification of ligand-receptor interactions
    • Principle | Dualsystems identify binding partners for Ligand-receptor interaction
    • Literature | TriCEPS-based ligand-receptor capture
  • Affinity and receptor expression | Assessment of the limits of the LRC-TriCEPS™ technology
  • Cell Types | Living cell types used with LRC-TriCEPS
  • Example of LRC-TriCEPS applied to human primary T-cells
  • Example of a LRC-TriCEPS experiment
  • Flow (Pretest) TriCEPS™ Kit |
  • News | Get here the latest news about Dualsystems Biotech custom screening services and products.
  • Blog | Blog article from Dualsystems.
  • Events | Events, trade fairs, partnering events Dualsytems plans to attend.
  • Company | Dualsystems Biotech is a provider of custom services to identify protein-protein interactions, including yeast two-hybrid services and our proprietary DUALmembrane and DUALhunter technologies for the identification of interactions involving membrane proteins and transcription factors. In addition, we provide kits and reagents for a range of research applications, including protein expression, protein purification and protein activity measurement.
  • Management of Dualsystems | Find more information about the Dualsystems Biotech management team.
  • Scientific Board | Find here more information about the Dualsystems Biotech scientific advisory board.
  • Investors | Are you interested in investment opportunities with Dualsystems Biotech?
  • Career | Are you interested in working for Dualsystems Biotech?
  • Contact | Get in contact with Dualsystems Biotech. Contact us by email, telephone or our online contact form and we will come back to you within 24 hours!
  • Bioinformatics tools | Bioinformatics tools

Investors

Investors


During two financing rounds in 2000 and in 2007 Dualsystems Biotech has built a solid investor base including private individuals as well as institutional investors to support our technology driven growth strategy.

Should you be interested in supporting us and become an investor in Dualsystems Biotech, please contact Dr. Paul Helbling, at subject line Investment.

Company

About Dualsystems Biotech


Dualsystems Biotech Schlieren Zurich Switzerland

Dualsystems Biotech is a provider of proteomics services for industry and academia. The LRC TriCEPS technology platform is designed to identify targets and off-targets irected to identify intracellular as well as extracellular drug targets. Our specialities LRC-TriCEPS™, ligand-receptor capture and FLOW-TriCEPS™ make use of mass spectrometry analysis of protein complexes.

  • Established in 2000
  • Facilities in Zurich, Switzerland
  • Provider of target screening technologies
  • Swiss Quality

Merken

Merken

Merken

dualsystems-biotech-building-schlieren

Contact

Contact


You are looking for the targets of your ligand (peptide, protein, antibody, and virus) that are relevant in the living organism?

Give us a call or fill in the form below and we get in contact to see how the LRC-TriCEPS™ (CaptiRec) technology can answer your question.

Send us mail

Dualsystems Biotech AG

Dualsystems Biotech AG
Dr. Paul Helbling
Grabenstrasse 11a
8952 Schlieren
Switzerland

phone +41 44 738 50 00
fax +41 44 738 50 05

Please contact Dr. Paul Helbling to discuss how we can support your projects.

dualsystems-zurich-du

Dualsystems Biotech is located in Schlieren (Zurich), close to the train station.