Drug profiling service procedure
Entry point
- Your drug of interest
Step 1 - Compound derivatization and toxicity test 
Duration: 4 — 8 weeks
- The small molecule is synthesized as a derivative linked to an anchor moiety
- The anchor moiety serves to couple the derivative to the bait scaffold and a flexible linker ensures that no sterical hindrance occurs
Optional: Synthesis may be performed by customer
Step 2 - cDNA library screen 
Duration: 2 — 4 weeks
- Selection of primary targets
- Identification of targets by sequencing and BLAST analysis
Step 3 - Clone selection and analysis
Duration: 2 — 4 weeks
- Selection of primary targets
- Identification of targets by sequencing and BLAST analysis
Step 4: Compound dependency assay
Duration: 1 — 2 weeks
The compound dependency assay selectively isolates targets which specifically interact with the small molecule derivative
Deliverables
- Focused list of specific drug targets
Follow up assays
- Competition assays
- In vitro binding assays (e.g. pulldown assays, Biacore studies)
- Validation of interactions in human cells
Do you need more information?
Please visit our new website for CRO services: www.drug-profiling.com
