Example of a CaptiREC (LRC-TriCEPS)
experiment performed at Dualsystems
to identify the target receptor of
Anti-EGFR-Antibody on human
breast cancer cells (MDA-MB-231)
TriCEPS™-based ligand-receptor capture (LRC-TriCEPS™) is a novel technology. LRC-TriCEPS™ enables the unbiased identification of cell surface receptors and off-targets on the living cells without genetic manipulation. Ligand-receptor capture technology can be used for many types of orphan ligands, such as peptides, proteins, antibodies, engineered affinity binders but also for viruses.
- Extracellular proteins
- Peptide ligands
- Engineered affinity binders
Dualsystems provides full analytical Services. After the method transfer a full LRC-TriCEPS™ Kit is available.
Do you want to do functional pretests with your ligand coupled to TriCEPS™?
Ask for the pretest TriCEPS™ Kit. Here
Unique service workflow produces complete data set. Identify all protein partners of your bait and monitor protein interactions in response to drugs / extra-cellular stimuli and map post-translational modifications based on affinity purification using a streptavidin tag.
Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are specialized in elucidating the interaction of the living cell with extracellular ligands using the ligand-receptor capture LRC-TriCEPS™ technology. For the intracellular signaling we provide the CaptiVate Services using affinity purification/mass spectrometry analysis of protein complexes. Further, we provide Shotgun Proteomics Services for proteins / peptides (LC-MS/MS services) and stable cell line generation for human (HEK, Jurkart), mouse NIH 3T3 and chinese hamster ovary (CHO) cells.