Example of a CaptiREC (LRC-TriCEPS)
experiment performed at Dualsystems
to identify the target receptor of
Anti-EGFR-Antibody on human
breast cancer cells (MDA-MB-231)
«The fruitful collaboration with Dualsystems
Biotech using the CaptiRec LRC-TriCEPS
technology showed that even on insect
cells receptors could be identified»
Alisson M. Gontijo, Principal Investigator at CEDOC
Centro de Estudos de Doenças Crónicas
"We have been trying to identify a receptor
that has been sought after for nearly 30
years by laboratories across the globe.
Thanks to Dualsystems service CaptiRec
(LRC-TriCEPS), we now have a strong
De'Broski R. Herbert Ph.D. Assistant Professor in Residence
University of California San Francisco (UCSF)
"Thanks to LRC-TriCEPS (CaptiRec)
technology we were able to identify a long
sought receptor of our ligand using
genetically-engineered cell lines with
the support of Dualsystems"
MD, PhD Samuel E. Schechter Professor of Medicine
Washington University School of Medicine
TriCEPS™-based ligand-receptor capture (LRC-TriCEPS™) is a novel technology. LRC-TriCEPS™ enables the unbiased identification of cell surface receptors and off-targets on the living cells without genetic manipulation. Ligand-receptor capture technology can be used for many types of orphan ligands, such as peptides, proteins, antibodies, engineered affinity binders but also for viruses.
- Extracellular proteins
- Peptide ligands
- Engineered affinity binders
Dualsystems provides full analytical Services. After the method transfer a full LRC-TriCEPS™ Kit is available.
Do you want to do functional pretests with your ligand coupled to TriCEPS™?
Ask for the pretest TriCEPS™ Kit. Here
Unique service workflow produces complete data set. Identify all protein partners of your bait and monitor protein interactions in response to drugs / extra-cellular stimuli and map post-translational modifications based on affinity purification using a streptavidin tag.
Dualsystems Biotech is a service provider for custom proteomics for industry and academia. We are specialized in elucidating the interaction of the living cell with extracellular ligands using the ligand-receptor capture LRC-TriCEPS™ technology. For the intracellular signaling we provide the CaptiVate Services using affinity purification/mass spectrometry analysis of protein complexes. Further, we provide Shotgun Proteomics Services for proteins / peptides (LC-MS/MS services) and stable cell line generation for human (HEK, Jurkart), mouse NIH 3T3 and chinese hamster ovary (CHO) cells.